Enzymatic Radioiodination of Gonadotropins
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چکیده
منابع مشابه
Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus.
Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this...
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A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and...
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Radioiodination with rz51 has emerged as the method of choice for labeling protein to high specific radioactivity. A number of procedures have been developed to iodinate proteins under mild conditions and with reproducible results (l-7). Of these techniques, none is suitable for the iodination of proteins having thiol groups. Exposure to the oxidants Nchloro-p-toluene sulfonamide (chloramine-T)...
متن کاملGonadotropins
This exceptionally well done book organizes the proceedings of the International Symposium on Gonadotropins held at the New York Hospital Bicentennial Celebration in 1971 into 58 essays which survey the chemistry and physiology of FSH, LH, and prolactin. The book's major attribute is the special care taken to present gonadotropin research in perspective. Thus, the first essay, if not all of the...
متن کاملGonadotropins
Five years after the demonstration of anterior pituitary dominance over the gonads by the Smiths and Aschheim and Zondek, Moore and Price asserted the classic principle of the reciprocal "feedback" relationship between the secretions of these organs. Three decades later continued investigation of this interdependence has culminated in two major advances in clinical medicine: steroid inhibition ...
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ژورنال
عنوان ژورنال: The Journal of Clinical Endocrinology & Metabolism
سال: 1972
ISSN: 0021-972X,1945-7197
DOI: 10.1210/jcem-34-1-23